![]() ![]() Click publication title for the full text. Please click the arrow on the right to expand the citation list. Related products: Universal Restriction Buffer, #EN-300 ![]() Supercoiled plasmids may require up to 5-fold more NotI for complete digestion than linear DNA.Īll preparations are assayed for contaminating endonuclease, 3'-exonuclease, 5' exonuclease/ 5' phosphatase, as well as nonspecific single- and doublestranded DNase activities. To obtain best results, consult the corresponding manuals of all involved products.Īfter 25-fold overdigestion with NotI, >98% of the DNA fragments can be ligated and recut with this enzyme. Our restriction enzymes are fully compatible to restrictases and buffer systems from other manufacturers and can be used along in double digestions. If optimum condition for second enzyme is different than the recommended for the first enzyme, we suggest carrying out first the restriction at the higher recommended concentration of UB and dilute the reaction volume to the adequate UB concentration for further proceeding with the second restriction. Within the Universal Buffer (UB) system, the most majority of our enzymes display 100% Relative Activity in 1x UB and only few either in 0.5x or 2x UB. Please note that the optimum digestion condition for this enzyme is 1x UB. Phenol-Chloroform Extraction or Ethanol Precipitation.ġx UB - 100 % Relative Activity (recommended) Gel Electrophoresis and Single Band Excision (e.g. Addition of 2.1 μl EDTA pH 8.0, final 20 mM After enzyme addition, mix gently by pipetting. Mix components well before adding enzyme.
0 Comments
Leave a Reply. |